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yclO [2017-11-16 13:27:58]
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yclO [2017-11-16 13:27:58]

petrobactin (3.4-catecholate siderophore) ABC transporter (permease)
locus
BSU03810
pI
9.73
mw
35.32 kDa
protein length
315 aa Sequence Blast
gene length
945 bp Sequence Blast
function
acquisition of iron
product
petrobactin ABC transporter (permease)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.5|Uptake of iron/ siderophores]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.1|ABC transporters for the uptake of iron/ siderophores]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.4|ABC transporters for the uptake of iron/ siderophores]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    433,315 → 434,262

    The protein

    Catalyzed reaction/ biological activity

  • uptake of the siderophore petrobactin [Pubmed|19955416]

    • Protein family

  • FecCD subfamily (according to Swiss-Prot)

    • [SW|Localization]

  • cell membrane [Pubmed|10092453]

    • Expression and Regulation

    Operons

    genes
    [gene|552D4C42DCEA4625000160D2625711C61AB11661|fpbN]-[gene|EC63B5CDFDB4DF3A967ECEC40F2C44849311148B|fpbO]-[gene|473628F86C18FE9957841F3C8B45242C90CB341D|fpbP]-[gene|C1D6D188E5BD039F32427B2A58BBFC0CC173A476|fpbQ]
    description
    [Pubmed|19955416]

    regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

    • regulation

  • immediately induced upon iron starvation (first wave to allow iron uptake) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]) [Pubmed|29133393,12354229]
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    Biological materials

    Mutant

  • MGNA-C066 (yclO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2064 NBRP B. subtilis, Japan]
  • BKE03810 (Δ[gene|EC63B5CDFDB4DF3A967ECEC40F2C44849311148B|fpbO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AACGAGCAAAGCTATTTTCA, downstream forward: _UP4_TTGCTGTTAAAGGAGAATAA
  • BKK03810 (Δ[gene|EC63B5CDFDB4DF3A967ECEC40F2C44849311148B|fpbO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AACGAGCAAAGCTATTTTCA, downstream forward: _UP4_TTGCTGTTAAAGGAGAATAA

    • References

  • 10092453,19955416,12354229